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Revision as of 15:47, 24 January 2022 by Jkinne (talk | contribs) (Additional Programs to Install)
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Additional Programs to Install

  • Compression - for those using Windows, install 7-Zip. MacOS and Linux natively support most compression formats that we will need.
  • R - first install R and then install RStudio Desktop (free).
  • Teams - ISU faculty/staff/students can install MS Teams for free along with other parts of Office 365. Start by logging into with your ISU credentials, click around looking for Teams (may have to click on "All Apps" or something like that), and once you get to Teams look for a link to download the desktop application (for Windows and Mac OS, not available for Linux). You can also use Teams in the browser.
  • Login to so you can be added onto projects there.


Potentially good things to read / tutorials, etc. ...

Foundations of Bioinformatics - UC San Diego CS (UCSC), Computational Biology - UT Dallas Dept. Biology

In particular, your assigned reading includes...

Gene Expression

Start by watching the video introduction (16min, watch it at 2x or 1.5x).

We start by getting into this GSE85331 dataset, described in this publication (and see supplementary information for how they processed/analyzed the data).

On your own computer, download the dataset and extract (uncompress) the file (on MacOS or Linux just double click it, on Windows use 7-Zip or something similar).


After extracting you can open the file in Excel, Sheets, or LibreOffice. Note that it is a tsv file. If you double click, your OS may not know what program to use to open it. So start your spreadsheet program and then open the file. Some things are not too painful to do in your spreadsheet program. For example, you should verify that the following are all correct...

  • Genes with highest H1_day0_0 values: SNORD97, SNHG25, EEF1A1, RPL38, RPS27.
  • Genes with highest H1_CM_0 values: H19, MYL7, RPL31, SNORD9, RPS27.
  • Number of genes (#rows - 1): 26257
  • Median value for H1_day0_0: 0.539942
  • Median value for H1_CM_0: 1.246015
  • Average value for H1_day0_0: 15.86772859
  • Average value for H1_CM_0: 16.4574767

It seems that this dataset might be normalized so that the average values for each column (sample) are similar.

And that is about all we want to do in the spreadsheet right now. You can save it as an xlsx or import into Google Sheets in case we want to do anything else manually with it.

R and R Studio

Start by watching the video about gse85331_first_look.R (18min).

First Look Let's see what we can do with the same file in R and R Studio. First you should install R and R Studio on your computer, see links above. Let's take a first look at the data and confirm the values we got from Excel. You can download the R file here - gse85331_first_look.R and run it to confirm this. See also [this video] showing the file and explaining it.

Differential expression From the supplementary information from the publication, differentially expressed genes were found as follows - "Statistical analysis was performed for each cell line individually by pairwise comparisons across time-points and day 0 (control)." So, let's see if we can duplicate that.